mhqO [2019-09-04 10:16:55]

hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone

Locus: BSU_05490

Isoelectric point: 4.83

Molecular weight: 34.90 kDa

Protein length: 312 aa

Gene length: 939 bp

Function: resistence to methyl-hydroxyquinone

Product: hydroquinone-specific dioxygenase

Essential: no

Synonyms: ydfO

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 597,114 598,052

The protein

Protein family

• extradiol ring-cleavage dioxygenase family (according to UniProt)

Paralogous protein(s)

• MhqA, MhqE

Structure

• 3OAJ

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: mhqO-mhqP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • MhqR: repression, PubMed, in MhqR regulon

  Regulation

  • induced by catechol and 2-methylhydroquinone (MhqR) PubMed

• Genes: mhqN-mhqO-mhqP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • MhqR: repression, PubMed, in MhqR regulon

  Regulation

  • induced by catechol and 2-methylhydroquinone (MhqR) PubMed

Biological materials

Mutant

• MGNA-C154 (ydfO::erm), available at the NBRP B. subtilis, Japan
• BKE05490 (mhqO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTAGCCATTTTTATCCGCT, downstream forward: _UP4_TGATTTGACACATAAAAAAT
• BKK05490 (mhqO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTAGCCATTTTTATCCGCT, downstream forward: _UP4_TGATTTGACACATAAAAAAT

References

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